Stock	Final	Amount
T7 10X Reaction Buffer	10X	1X	50 μL
T7 ATP Solution	75 mM	7.5 mM	50 μL
T7 CTP Solution	75 mM	7.5 mM	50 μL
T7 GTP Solution	75 mM	7.5 mM	50 μL
T7 UTP Solution	75 mM	7.5 mM	50 μL
T7 FP1 (sequence is shown below)	100 μM	0.6 μM	3 μL
DNA library above		0.5 μM	250 pmol
T7 Enzyme Mix			50 μL
Add water to 500 μL