Stock	Final	Amount
Transcription Buffer	5X	1X	200 μL
PEG-8000	400 mg/mL	50 mg/mL	125 μL
MgCl2	1 M	25 mM	25 μL
ATP	100 mM	4 mM	40 μL
CTP	100 mM	4 mM	40 μL
GTP	100 mM	4 mM	40 μL
UTP	100 mM	4 mM	40 μL
Inorganic pyrophosphatase	0.1 U/μL	2.5 U/mL	25 μL
PCR-amplified DNA		75 nM	75 pmol
T7 RNA polymerase		0.05 μg/μL	50 μg
Add water to 1000 μL