| Stock | Final | Amount | |
|---|---|---|---|
| XL buffer | 10X | 1X | 175 μL |
| PAGE-purified library RNA | 3 μM | 5250 pmol | |
| XL-PSO | 100 μM | 7.5 μM | 131.25 μL |
| Add water to 1750 μL |
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