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. 2018 May 24;13(5):e0198039. doi: 10.1371/journal.pone.0198039

Fig 1. Experimental protocol.

Fig 1

(A) D-glucose was injected intraperitoneally at 15 min before 1-h transient middle cerebral artery occlusion (MCAO). Following MCAO, the mice were re-perfused for 2 h and then injected with recombinant tissue plasminogen activator (tPA, n = 7), phosphorylated recombinant heat shock protein 27 (prHSP27, n = 6), tPA plus prHSP27 (tPA+prHSP27, n = 7), or bovine serum albumin (BSA, n = 9). The mice were sacrificed 24 h later. (B) Representative photomicrographs of infarct areas stained with cresyl violet at 24 h after reperfusion. Infarct areas are circumscribed with dotted lines. prHSP27 decreased brain infarct size. Scale bar = 2 mm. (C) Brain swelling. (D) Infarct volume corrected for edema. *p < 0.05, §p < 0.001. (E) prHSP27 improved neurological severity scores at 1 h following MCAO and at 24 h following reperfusion in mice treated with the above substances. Data are mean ± standard error of the mean (SEM; n = same as above). *p < 0.05: tPA and prHSP27 vs. tPA, §p < 0.001: BSA vs. prHSP27.