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. 2018 May 24;13(5):e0198039. doi: 10.1371/journal.pone.0198039

Fig 5. Effect of prHSP27 on the expression of matrix metalloproteinase-9 (MMP-9) on the ischemic side.

Fig 5

(A) Zymographic (upper) and densitometric (lower) analyses of MMP-9 protein in BSA- (n = 7), tPA- (n = 7), and tPA plus prHSP27- (n = 7) treated mice at 24 h after reperfusion. ‡p < 0.005: tPA plus prHSP27 vs. tPA. (B) Photomicrographs of ionized calcium binding adapter molecule-1 (Iba-1) immunostaining (upper) and number of Iba-1-positive cells (lower) in the infarct boundary zones in BSA- (n = 5), tPA- (n = 5), and tPA plus prHSP27- (n = 5) treated mice at 24 h after reperfusion. prHSP27 suppressed the inflammatory response. Scale bars = 100 μm. Data are mean ± SEM. *p < 0.05: tPA plus prHSP27 vs. BSA, §p < 0.001: tPA plus prHSP27 vs. tPA.