Figure 4.
Analyses of RNA Sequencing Data of BAT of WT and ERRαγAd−/− Mice Treated with CL316,243
(A) Heat maps of genes significantly induced (p < 0.05) by CL316,243 in WT and ERRαγAd−/− BAT, using a 1.5-fold cut-off. Minimum and maximum log transformed values are −9 and 6, respectively, with visual contrasts set to 2. (i) Genes induced significantly by CL316,243 in WT BAT only. (ii) Genes induced by CL316,243 in both WT and ERRαγAd−/− BAT, but expression is significantly higher in WT CL316,243 vs. ERRαγAd−/− CL316,243. (iii) Genes induced by CL316,243 in both WT and ERRαγAd−/− mice. (iv) Genes induced by CL316,243 in ERRαγAd−/− BAT only.
(B) Venn diagram of genes significantly induced (p < 0.05) by CL316,243 in BAT. Sections i–iv correspond with genes displayed in heat maps (A).
(C) Heat maps of genes significantly reduced (p < 0.05) in ERRαγAd−/− vs. WT BAT, in PBS- and/or CL316,243-treated mice, using a 1.5-fold cut-off. Minimum and maximum log transformed values were −5 and 6, respectively, with visual contrasts set to 2. (i) Genes reduced in PBS-treated mice only. (ii) Genes reduced in both PBS- and CL316,243-treated ERRαγAd−/− mice. (iii) Genes reduced in CL316,243-treated ERRαγAd−/− mice only.
(D) Venn diagrams of genes significantly reduced (p < 0.05) in ERRαγAd−/− BAT. Sections i–iii correspond with genes displayed in heat maps (C).
Male mice were born and raised at thermoneutrality (30°C), treated with PBS or CL316,243 for 10 days, and euthanized at 12 weeks of age (n = 3). See also Figure S4 and Tables 1, S1, and S2.