Figure 6.

Metabolic Responses to Adrenergic Stimulation in WT and ERRαγ^Ad−/− Mice

(A) Oxygen consumption (VO₂) in WT and ERRαγ^Ad−/− mice injected with PBS for 10 days, on the last day of injections.

(B) Core body temperature of WT and ERRαγ^Ad−/− mice injected with PBS for 10 days, on the last day of injections.

(C) Average respiratory exchange ratio (RER) over 6 hr following a PBS or CL316,243 injection.

(D) VO₂ in WT and ERRαγ^Ad−/− mice injected with CL316,243 for 10 days, on the last day of injections.

(E) Core body temperature of WT and ERRαγ^Ad−/− mice injected with CL316,243 for 10 days, on the last day of injections.

(F) Average VO₂ over the day and night on the day after the last PBS or CL316,243 injection (day, 20–32 hr after last injection; night, 32–44 hr after the last injection).

(G) Acute VO₂ response to norepinephrine (NE) in WT and ERRαγ^Ad−/− mice that had been treated with PBS for 10 days.

(H) Acute VO₂ response to NE in WT and ERRαγ^Ad−/− mice that had been treated with CL316,243 for 10 days.

Male mice were born and raised at thermoneutrality (30°C), treated with PBS or CL316,243 for 10 days, and euthanized at 12–14 weeks of age. NE responses were tested 2 days after the last PBS or CL316,243 injection. Data are mean ± SEM (n = 5–10). *p < 0.05, **p < 0.01, ***p < 0.001, WT vs. ERRαγ^Ad−/− for each condition; ^#p < 0.05, ^##p < 0.01 vs. WT PBS. ZT = zeitgeber time. Different mice were used for calorimetry and telemetry experiments. See also Figure S6.