Fig 7. Infection with SRL1 in Il17ra KO mice following depletion of neutrophils.

Il17ra KO mice were infected with 10⁶ cfu/mouse of SRL1by intranasal inoculation 24 hours after intraperitoneal inoculation of 500 μg antibody 1A8 (anti-Ly6G) or isotype matched control and observed for the course of the infection. (A) peripheral blood neutrophil counts following treatment with isotype control (filled circles) or anti-Ly6G (open circles) at 24 hours following infection. Each point is from an individual animal; line indicates median. Difference is significant (**, p < 0.01, Mann Whitney test) (B). Kaplan Meir plot of survival of infected mice. Isotype control treated mice (solid line) had improved survival compared to neutrophil depleted mice (dashed line) (p = 0.017, log rank test). (C) clinical score between isotype treated mice (solid circles) and neutrophil depleted mice (open circles) Differences are significant between the groups (p < 0.0001, 2-way ANOVA).