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. 2018 May 29;14(5):e1007070. doi: 10.1371/journal.ppat.1007070

Fig 4. Effect on KSHV late mRNA accumulation of DNA replication blockade at various times after initiation of lytic replication.

Fig 4

The effect of blocking DNA replication after induction on the subsequent accumulation of late gene mRNAs was assessed in KSHV infected cells. A-D. RNA accumulation at 48 hr post induction. iSLK cells were treated with doxycycline to induce lytic KSHV replication. RNA was harvested at different time points up to 48 hr as shown after induction at the left of each panel. DNA replication was also blocked at various times after induction (+PAA), RNA synthesis was allowed to proceed, and RNA was harvested at 48 hr, as shown at the right of each panel. qPCR was performed to measure relative quantity (RQ) of each lytic early gene [ORF59, ORF57] and late gene (ORF25, ORF26] as shown. E-F. RNA accumulation at 96 hr post induction. iSLK cells were treated with doxycycline and PAA as indicated. RNA was harvested at different time points up to 96 hr after induction (harvest), shown at the left of each panel. After blockade of DNA replication with PAA as in A-D above, RNA was harvested at 96 hr and qPCR was performed to measure relative quantity (RQ) of each lytic late gene (ORF25, ORF26]. Error bars represent SEM.