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. 2018 May 29;14(5):e1007070. doi: 10.1371/journal.ppat.1007070

Fig 5. Effect of serial replication blockade on EBV lytic mRNA accumulation.

Fig 5

The effect of blocking DNA replication after induction on the subsequent accumulation of late gene mRNAs was assessed in EBV infected cells. AGSiZ cells were doxycycline (+D) treated to induce lytic replication. RNA was harvested at different time points after induction up to 24 hr post-induction as shown at the left of each panel. PAA was added to block DNA replication at 0, 6, 12 and 18 hr, RNA synthesis was allowed to proceed, and RNA was harvested at 24 hr, as shown at the right of each panel. qPCR was performed to measure relative quantity (RQ) of each lytic early gene [BMRF1, SM] (C,D) and late gene (BDLF1, BcLF1] (A,B) as shown. Error bars represent SEM.