. 2018 May 29;14(5):e1007106. doi: 10.1371/journal.ppat.1007106

Table 1. CspA variants differ in binding to factor H from different animals.

CspA variant	Factor H source	ELISA K_D (μM)^b	-----Surface Plasmon Resonance----
CspA variant	Factor H source	ELISA K_D (μM)^b	K_D (μM)^a	k_on (10⁵s^-1M^-1)	k_off (s^-1)
B. burgdorferi
CspA_B31	Human	0.41±0.09	0.20±0.07	1.14±0.42	0.017±0.005
	Mouse	0.23±0.08	0.07±0.01	1.88±0.36	0.012±0.005
	Horse	0.88±0.33	0.55±0.18	0.30±0.01	0.014±0.003
	Quail	0.92±0.28	n.d.^a	n.d.	n.d.
CspA_B31L246D	Human	n.s.^b	n.b.	n.b.	n.b.
	Mouse	n.s.	n.b.	n.b.	n.b.
	Horse	n.b.^c	n.b.	n.b.	n.b.
	Quail	n.b.	n.d.	n.d.	n.d.
DbpA_B31^d	Human	n.b.	n.d.	n.d.	n.d.
	Mouse	n.b.	n.d.	n.d.	n.d.
	Horse	n.b.	n.d.	n.d.	n.d.
	Quail	n.b.	n.d.	n.d.	n.d.
B. afzelii
CspA_Pko	Human	0.16±0.05	0.11±0.02	1.52±0.62	0.014±0.004
	Mouse	0.76±0.64	0.46±0.14	1.15±0.31	0.048±0.011
	Horse	n.b.	n.b.	n.b.	n.b.
	Quail	n.b.	n.d.	n.d.	n.d.
B. garinii
CspA_ZQ1	Human	n.b.	n.b.	n.b.	n.b.
	Mouse	n.b.	n.b.	n.b.	n.b.
	Horse	n.b.	n.b.	n.b.	n.b.
	Quail	0.36±0.13	n.d.	n.d.	n.d.

All values represent the mean ± SEM of three experiments.

^aNot determined.

^bNot saturated

^cNo binding activity was detected.

^dDbpA_B31 was included as a negative control.