Table 1. Time intervals of the different mitotic and cytokinetic events at 28°C.
| Strain | Prophase1 | Metaphase/Anaphase A1 | Anaphase B1 | |
|---|---|---|---|---|
| Septation onset2 | Spindle disassembly2 | |||
| Wild-type (n = 18, 126 cells)3 | 6.5 ± 1.2 | 5.9 ± 0.5 | 5.7 ± 1.0 (18.1)4 | 7.1 ± 1.1 (25.2)4 |
| cdc13+-GFP (n = 3, 20 cells) | 5.5 ± 0.5 | 6.2 ± 0.4 | 5.7 ± 0.8NS (17.4) | 5.2 ± 1.2 (22.6) |
| cdc15-GFP (n = 4, 14 cells) | 6.3 ± 0.9 | 6.3 ± 1.5 | 11.2 ± 1.4*** (23.8) | 3.4 ± 1.3 (27.2) |
| sid2-250 (n = 2, 30 cells) | n.d. | n.d. | 9.1 ± 1.1*** (n.d.) | 3.5 ± 2.3 (n.d.) |
| myp2Δ (n = 2, 10 cells) | 6.5 ± 0.5 | 6.8 ± 1.7 | 7.4 ± 0.8*** (20.7) | 6.1 ± 1.6 (26.8) |
Values are minutes ± SD.
1 Mitotic phases are as described in Fig 1A.
2 Elapsed time between septation onset and steps of anaphase B.
3 The value n is the number of independent experiments performed in different days in each case.
4 The values underlined in parenthesis are the total minutes since the mitotic entry in prophase.
Asterisks indicate significant statistical difference in the septation onset of the corresponding strain compared to wild-type by the Student´s test:
* p <0.05
** p <0.01
*** p <0.001; NS: not significant difference (p >0.05).
n.d., not determined.