Table 3. Spindle length at septation onset with respect to the cell size and the maximal spindle length.
| Strain | Spindle length at septation onset1 | Cell length2 | Septation / cell size ratio3 | Spindle length at late anaphase4 | Septation / spindle length ratio5 |
|---|---|---|---|---|---|
| 28°C | |||||
| Wild-type (17 cells) | 7.8 ± 0.7 μm | 13.9 ± 0.8 μm | 55.9 ± 3.8 (%) | 10.8 ± 0.6 μm | 71.3 ± 5.7 (%) |
| cdc13-GFP (15 cells) | 7.9 ± 0.9 μm | 14.1 ± 1.0 μm | 55.9 ± 4.7 (%) | 11.3 ± 0.9 μm | 69.9 ± 6.3 (%) |
| cdc15-GFP (11 cells) | 11.0 ± 0.6 μm | 15.3 ± 1.1 μm | 72.2 ± 5.9 (%) | 11.4 ± 0.6 μm | 96.4 ± 2.7 (%) |
| sid2-250 (11 cells) | 10.6 ± 0.7 μm | 15.2 ± 0.7 μm | 70.0 ± 4.8 (%) | 12.1 ± 0.7 μm | 87.6 ± 7.2 (%) |
| 25°C | |||||
| Wild-type (15 cells) | 7.9 ± 0.9 μm | 14.7 ± 0.8 μm | 53.7 ± 5.9 (%) | 11.5 ± 0.9 μm | 69.0 ± 6.4 (%) |
| cdc25-22 (10 cells) | 14.8 ± 1.6 μm | 37.8 ± 3.9 μm | 39.2 ± 3.6 (%) | 21.1 ± 2.2 μm | 70.4 ± 6.9 (%) |
| cdc10-119 (9 cells) | 11.1 ± 0.7 μm | 28.3 ± 3.5 μm | 39.4 ± 2.6 (%) | 17.6 ± 1.7 μm | 64.3 ± 3.4 (%) |
| Wild-type diploid (16 cells) | 12.2 ± 1.3 μm | 21.5 ± 1.8 μm | 57.2 ± 5.4 (%) | 14.6 ± 0.8 μm | 84.1 ± 7.8 (%) |
| wee1-50 (13 cells) | 5.1 ± 0.7 μm | 10.2 ± 0.8 μm | 50.2 ± 6.1 (%) | 8.7 ± 0.6 μm | 58.3 ± 10.3 (%) |
| cdc2-3W (17 cells) | 5.2 ± 0.7 μm | 9.3 ± 1.1 μm | 55.6 ± 7.9 (%) | 8.3 ± 1.1 μm | 61.2 ± 9.4 (%) |
Values are mean ± SD.
The distance between chromosome masses was measured in cells randomly selected from the experiments shown in Table 1 (28°C) and Table 2 (25°C).
1 Length between chromosome masses (= spindle length) at septation onset.
2 Cell length at septation onset.
3 Percentage of spindle length at septation onset with respect to the total cell length.
4 Maximal length between separated chromosome masses (= spindle length) at late anaphase.
5 Percentage of spindle length at septation onset with respect to the maximal spindle length at the end of anaphase.