Fig 7. D-[U-¹⁴C]-glucose incorporation into lipids of the EATRO1125.T7T (PCL) and Δach/^RNAiASCT cell lines.

In this experiment, trans-esterification of fatty acids was not performed before HPTLC separation and the different fatty acid-containing lipids were combined as performed before [10]. Data are normalized with the parental cell (PCL) values with an arbitrary value of 100 for the PCL samples, which is represented by a horizontal dashed lane. Black columns correspond to the EATRO1125.T7T parental cell line, grey columns to the non-induced (.ni) mutant Δach/^RNAiASCT cells, and the light grey and white columns to the mutant cells tetracycline-induced for 8 days (.i 8d) or 15 days (.i 15d), respectively. It is to note that the SDM79 used for this labelling experiment does not contain acetate. Error bars indicate mean ± SD of 3 biological replicates. The inset shows a western blot control using the anti-ASCT and anti-hsp60 immune sera.