Fig 9. IVDH is needed for growth of PCF in medium depleted in ketogenic carbon sources.

The figure shows growth curve of the parental cell line (PCL, black curve), the Δivdh-C3 (ΔC3, blue curve) and Δivdh-C8 (ΔC8, red curve) null mutants, as well as the Δivdh-C3/IVDH rescue cell line (R, grey curve) incubated in SDM79 medium containing or not 4 mM of glucose (G), threonine (T) and acetate (A), and supplemented with normal FCS (solid lines) or delipidated FCS (dashed lines). Since threonine is required for protein biosynthesis, 150 μM of this amino acid is present in the glucose/threonine/acetate-depleted conditions. Cells were maintained in the exponential growth phase (between 10⁶ and 10⁷ cells/ml) and cumulative cell numbers reflect normalization for dilution during cultivation. The inset shows western blot analysis of these cell lines with the anti-IVDH and anti-PFR immune sera.