Fig. 2.

Forskolin has radical scavenging activity. a) Free radical scavenging activity of the test compounds (DOX − 2 μM, MET-1 μM, FSK-10 μM) was measured using DPPH assay and spectrophotometry. *P < 0.05 when compared with the respective control. Ascorbic acid (AscA1–3.125 μg/ml, AscA2–6.25 μg/ml, AscA3–12.5 μg/ml) was used as a positive control. b) Forskolin attenuates doxorubicin-induced reactive oxygen species production. THP1 cells were incubated with the test compounds (DOX- 2 μM, MET-1 μM, FSK-10 μM) at 37 °C, 5% CO₂ for 24 h. Treated cells were labeled with DCFDA (20 μM) to measure ROS generation by flow cytometry. Three controls comprising THP1 cells alone, DCFDA alone and H₂O₂ control were included in the assay. M3 represents normal ROS production in THP1 cells, M2 represents H₂O₂ control and M1 represents compound(s)-induced ROS production.