Fig. 3.

Measurement of SAH concentration through SAM-to-SAH conversion assay. a) Calibration curve for reference SAH. b) Cells were incubated with doxorubicin (DOX, 2 μM), forskolin (FSK, 10 μM) and Metformin (MET, 1 μM), in specific combinations, at 37 °C, 5% CO₂ for 24 h. Quantification was performed as a homogenous time resolved fluorescence (HTRF) assay using multimode plate reader at 665 or 620 nm. Data demonstrates the effect of test compounds on the SAM-dependent methyltransferase activity through intracellular SAH concentrations. Results are the mean ± SD of three measurements. *-significantly different at P < 0.05 as compared with the respective control.