Figure 2.

Expression of inhibitory receptors on CD8⁺ CTL, CD4⁺ Th and CD4⁺ Treg in the tumor, TFL and blood of MMR-proficient LM-CRC. PBMC and leukocytes isolated from LM-CRC tumors and TFL were stained with antibodies against PD-1, LAG3, TIM3 and CTLA4. (A) (B) Representative dot plots of inhibitory receptor expression on (A) CD3⁺CD8⁺ CTL and (B) CD3⁺CD4⁺Foxp3⁻ Th in the tumor, TFL and blood; the gates were made according to appropriate isotype controls. (C) (D) (E) The frequencies of inhibitory receptor positive cells within (C) CD8⁺ CTL, (D) CD4⁺Foxp3⁻ Th and (E) CD4⁺Foxp3⁺ Treg in the tumor, TFL and blood. Values of individual patients are shown, and lines depict medians. Differences were analyzed by paired t test or Wilcoxon matched pairs test; *p < 0.05, **p < 0.01, ***p < 0.001.