Table 3.
List of primers used.
| Primers | Sequence 5 ´to 3´ | Annealing temperature (degree celsius) | Company (City, Country) |
|---|---|---|---|
| B2M Exon 1 F | GGCATTCCTGAAGCTGACA | 59 | Life Technologies (Darmstadt, Germany) |
| B2M Exon 1 R | AGAGCGGGAGAGGAAGGAC | 59 | Life Technologies (Darmstadt, Germany) |
| B2M Exon 2a F | TTTTCCCGATATTCCTCAGGTA | 57 | Life Technologies (Darmstadt, Germany) |
| B2M Exon 2a R | AATTCAGTGTAGTACAAGAG | 57 | Life Technologies (Darmstadt, Germany) |
| B2M Exon 2b F | CATTCAGACTTGTCTTTCAG | 64 | Life Technologies (Darmstadt, Germany) |
| B2M Exon 2b R | TTTCAGCAGCTTACAA | 64 | Life Technologies (Darmstadt, Germany) |
| NLRC5 F | CTCCTCTCACCCTCTCCTCT | 60 | biomers.net (Ulm, Germany) |
| NLRC5 R | GCAGCCCCTACTTACCTGAT | 60 | biomers.net (Ulm, Germany) |