Figure 7.

Modulation of the cytokine profile in HCC immune-infiltrating cells by omeprazole treatment. (A-D) Multiparametric flow cytometry of live cells in freshly dissociated HCC tumors treated with the drug vehicle (red line) or with omeprazole (100 μg/ml, blue line) for 24 h. IFNγ, TNF and CCL22 production was evaluated by intracellular staining (A) in CD163⁺ myeloid cells gated inside live CD45⁺CD3⁻ cells and (B) in CD3⁺ T cells gated inside live CD45⁺ cells. FMO control for each cytokine (gray line) is reported in the histogram plot. The percentages of IFNγ-, TNF- and CCL22-positive cells in the CD163⁺ and in the CD3⁺ cells are shown in C and D respectively. The p-values are calculated by the paired t test comparing cells treated with omeprazole or the vehicle.