Figure 1.

CD71 is expressed on MOC1 cells and endothelial cells within the tumor microenvironment. A, MOC1 cells were assayed for CD71 expression by flow cytometry (clone R17217; left panel) or immunofluorescence (right panel). B, MOC1 tumors were harvested, sectioned, and assessed for CD71 expression via immunofluorescence (40x image, H&E on left). C, MOC1 tumor or oral mucosa was harvested, digested into a single cell suspension and assessed for CD71 expression on CD45.2⁻CD31⁻ epithelial/tumor cells, CD45.2⁺CD31⁻ immune cells or CD45.2⁻CD31⁺ endothelial cells via flow cytometry. Representative dot plots of the digested tissues on the left, with representative CD71 histograms and quantification (MFI, n = 5 mice) on the right for each cell type. **, p < 0.01; ***, P,0.001.