Figure 4.

Induction of IFN-dependent immunogenicity following scL-53 treatment is STING dependent. A, MOC1 cells were treated with scL loaded with TMEM173 siRNA (100 ng nucleic acid per 1 × 10⁴ cells) or scL-empty (volume equivalent) for 4 hours and then incubated for 24 hours. TMEM173 protein expression was assessed via intracellular flow cytometry. B, following treatment as in A, MOC1 cells were treated with 20 μM R,R-CDA synthetic cyclic dinucleotide for 24 hours and IFNβ production was assayed by intracellular flow cytometry. C, following treatment as in A, MOC1 cells were treated with either scL-53 or scL-vec for 4 hours, then incubated for 24 hours. IFNβ production was assayed by intracellular flow cytometry. D, following treatment as in A, MOC1 cells were treated with either scL-53 or scL-vec for 4 hours, then incubated for 24 hours. Expression of cell surface components of immunogenicity was assayed via flow cytometry. **, p < 0.01; ***, P,0.001.