Figure 1.

MG1-Maraba lethally infects prostatic cancer. Relative viability of multiple PCa cell lines was assessed using alamar blue 48 hours after infection of PCa cells in monolayer over a range MOIs (mean and SEM displayed, assays performed in triplicate) (A). Representative image of PCa core biopsies being obtained following prostatectomy for the treatment of a patient diagnosed with advanced PCa (B). Plaque assays following ex vivo infection from 10 patient's PCa core biopsies with MG1-GFP (mean and SEM displayed, assays performed in duplicate) (C). Representative bright field, fluorescent and overlaid images following infection of a core biopsy with MG1-GFP (4x magnification) (D). Immunohistochemical quantification of % cell positivity for MG1-Maraba following ex vivo infection of PCa biopsy cores with MG1-GFP (mean and SEM displayed, all sections stained at least in duplicate except patient #7 where biopsy material was limited) (E). Representative image of immunohistochemical staining for MG1-Maraba using DAB substrate chromogen and hematoxylin counterstain (F) (scale bar = 900 μm).