Figure 3.

Oncolytic vaccination breaches immune tolerance. Tumor-free female C57BL/6 mice were treated with oncolytic vaccination against STEAP (n = 3) to assess the induction of specific immune responses against known STEAP peptide epitopes as indicated by IFNγ production from CD8+ T cells collected after the prime (A) and boost (B) (mean and SEM displayed). Tumor-free male mice were treated with oncolytic vaccination against STEAP (n = 5) as well as a control group of mice treated with Ad-BHG: MG1-GFP (n = 5). ICS was performed after prime (C) and boost (D) (mean and SEM displayed). Prostatic inflammation was observed following STEAP vaccination (E) (hematoxylin and eosin)- note the heavy pleocelluar luminal infiltrate compared to healthy prostatic tissue (F) (hematoxylin and eosin). Luminal epithelial STEAP expression was confirmed immunohistochemically in the healthy prostate (G) (DAB substrate chromogen and hematoxylin counterstain) (scale bars = 100 μm).