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. 2018 Mar 26;7(7):e1445457. doi: 10.1080/2162402X.2018.1445457

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Figure 6. — Combined immunotherapy with vaccine and ultra-low dose paclitaxel downregulates MDSC and Treg activity markers. Tumor-infiltrating MDSC and Treg from RET (A-D, n = 8) and BRAF mice (E-H, n = 8) were analyzed by flow cytometry 10 days upon last vaccination. Arg-1 expression (A, E) by MDSC is presented as MFI. (B, F) PD-L1 expression is shown as the percentage of positive cells within total MDSC. (C, G) CD4⁺CD25⁺Foxp3⁺ Treg are presented as the percentage within total CD4 T cells. (D, H) Ki67 expression on Treg is shown as the percentage of respective subset among total Treg. Data are representative of two independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.