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. 2018 Mar 26;7(7):e1445457. doi: 10.1080/2162402X.2018.1445457

Figure 7.

Figure 7.

Enhanced anti-tumor effect of DC vaccine and anti-PD-1 antibodies in melanoma-bearing mice. RET-tg mice with established tumors were vaccinated i.p. three times in weekly intervals using DC electroporated with combination of mRNA encoding for hgp100 + TRP-2 + TRP-1 + TYR- hβ2 m-Kb/TLR4 (Q-Mix). Some vaccinated mice additionally received 4 times i.p. injections of 250 µg anti-PD-1 antibody (aPD-1) or isotype control (Control) every 3 days. (A) Survival of mice is shown as a Kaplan-Meier curve (n = 6–12/group). Ten days after last vaccination, tumor infiltrating CD8 T cells were analyzed by flow cytometry (n = 8). Results are presented as the percentage of tumor-infiltrating CD8 T cells among total leukocytes (B), MFI of the TCR ζ-chain in CD8 T cells (C), the percentage of Ki67+ (D), CD69+ (E), CD62 L−CD44+ EM (F) and IFN-γ producing CD8 T cells (G) among total CD8 T cells. Data are representative of two independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.