Figure 7.

Increased reversion of the immunosuppressive microenvironment by MbDox+US treatment. A. Splenic monocytes from mice injected with indicated formulations were stained with anti-CD8 antibody and an anti-IFN-γ (intracellular) antibody, and then analyzed with flow cytometry. Representative images are shown. B. Percentages of IFN-γ-expressing CD8⁺ T cells analyzed by flow cytometry in LL/2 and CT26 tumor models. C. Tumor-infiltrating lymphocytes (TILs) from tumor tissues of both LL/2 and CT26 were analyzed by flow cytometry. Representative images the IFN-γ-producing CD8⁺T cells are shown. D. Percentage of the IFN-γ-producing CD8⁺ T cells in TILs from mice of LL/2 and CT26 models. E. CD4⁺ T cells in TILs were gated out and detected as CD25⁺ and FOXP3⁺ double-positive Treg cells by flow cytometry. Representative images of the CD4⁺CD25⁺FOXP3⁺ Treg cells are shown. F. Percentage of CD4⁺CD25⁺FOXP3⁺ Treg cells in TILs from mice of LL/2 and CT26 models. Data are expressed as mean ± SEM, * indicates P < 0.05, ** indicates P < 0.001.