Figure 3.
TH Transporter Expression in SCs
(A) Fold changes based on log2-transformed RNA values from quiescent SCs, cultured myoblasts, myocytes, and myotubes were used to generate global ratio heat maps. While Lat1 is highly expressed in all cell types, Mct8 and Mct10 exhibit strongest expression in SCs. Oatp1c1 and Lat2 mRNA levels are low compared with Mct8.
(B) Primary EDL myofibers isolated from female WT mice and cultured for 0, 42, or 72 hr.
Immunofluorescence studies revealed MCT8 expression (in green, arrow) in PAX7-positive (red) SCs at all analyzed time points. In comparison, OATP1C1 (in green, arrow) could not be detected at 0 hr but was clearly visible after 42 and 72 hr in PAX7-positive (in red) SCs. Nuclei stained in blue. Representative images of n = 3. Scale bar: 5 μm.