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. 2018 May 17;10(6):1895–1907. doi: 10.1016/j.stemcr.2018.04.015

Figure 1.

Figure 1

GATA6 Is Expressed in a Small Subset of hESCs

(A) Representative FACS plot of the Shef4 GATA6-GFP reporter line S4G6 A3 cultured in KO/SR and MEF conditions. Black peak represents the unmodified parental Shef4 control line, and red, the Shef4 GATA6-GFP reporter line.

(B) Representative FACS plot of SSEA3 vs GATA6 expression. Left panels show gating controls P3X (above) and TRA-1-85 (below) on the Shef4 parental line. Right panel shows the identification of distinct cell populations: SSEA3 high, GATA6 negative (3+/6−); SSEA3 high, GATA6 low (3+/6L); SSEA3 high, GATA6 high (3+/6H); SSEA3 negative GATA6 high (3−/6+), of the GATA6 reporter line.

(C) Representative FACS plots of additional stem cell surface markers, SSEA3, TRA-1-81 or SSEA4 vs GATA6 expression with the same controls as (B).