BAF Complexes Globally Control Epigenetic and Gene Expression Programs in Late Development Pallium
(A) Table showing the peptide number for KDM6A, KDM6B, and KDM1A proteins purified from BAF155 and BAF170 immunoprecipitates of protein extracts from NS5 cells, E13.5 or E17.5 forebrain.
(B) Interactions of BAF155 and BAF170 with KDM6A, KDM6B, and KDM1A were confirmed by coIP/western blot (WB) analyses of E17.5 pallium tissue.
(C–E) Distribution of H3K27me3 (C) and H3K4me2 (D) marks along gene bodies in the dcKO and control pallium at E17.5. H3K27me3 levels are increased in dcKOs. dcKO (E) genes with increased H3K4me2 or H3K27me3 marks in the dcKO pallium at E17.5 are largely non-overlapping.
(F) Volcano plot representing differentially regulated genes in the dcKO pallium at E17.5. Experimental replicates (n) = 4 (C, D; ChIP-seq), 4 (control for RNA-seq; F), 3 (dcKO_hGFAP-Cre for RNA-seq; F).