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. 2018 May 17;10(6):1734–1750. doi: 10.1016/j.stemcr.2018.04.014

Figure 2.

Figure 2

H3K27me3-Linked Silencing of Neuronal Differentiation-Related Genes in BAF Complex-Deleted Pallium in Late Stages

(A) Neuronal differentiation-related genes are downregulated in the dcKO pallium at E17.5.

(B) General H3K27me3 profile plot of neuronal differentiation-related genes that are downregulated in dcKO pallium.

(C) Upper panel: heatmap depicting the changes in H3K27me3 levels at neural differentiation-related genes that are downregulated in dcKO pallium at E17.5 individually. Lower panel: average relative H3K27me3 binding levels on those genes combined.

(D) Integrated genome browser views of H3K27me3 and BRG1 (GEO: GSE37151; Attanasio et al., 2014) binding along representative neural differentiation-related genes downregulated in dcKO pallium.

(E–G) IF (E) and quantitative (F and G) analyses indicate that the loss of BAF155 and BAF170 leads to a diminished thickness of the HUCD+ cortical plate (CP) and intermediate zone (IZ) (F), and expanded thickness of the HUCD− VZ (G) in the entire pallium at E15.5–E18.5.

Values are presented as means ± SEMs (∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.005; ∗∗∗∗p < 0.0001). Experimental replicates (n) = 6 (F and G). Abbreviations: VZ, ventricular zone; CP, cortical plate; IZ, intermediate zone; MP, medial pallium; DP, dorsal pallium; LP, lateral pallium. Scale bar represents 100 μm (E).