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. 2018 May 17;10(6):1734–1750. doi: 10.1016/j.stemcr.2018.04.014

Figure 7.

Figure 7

BAF Complexes Control Hippocampal Development by Suppressing Wnt Signaling Activity

(A) Schematic model of the molecular cascades underlying late stages of pallium development in WT and dcKO pallium.

(B) Rescue experimental paradigm with the Wnt inhibitor (WNTi, ICG-001).

(C–E) IF (C and D) and quantitative (E) analyses of dcKO mutants at the indicated stages, showing the effects of treatment with ICG-001 on pools of PAX6+ NSCs (C and E), CASP3+ apoptotic cells (C and E), and ZBTB20+ (D and E) and PROX1+ neurons (E) in the developing hippocampus.

(F and G) Expression of Wnt (F) and Proliferation (G)-related genes in control, vehicle (Veh), WNTi-treated pallium.

(H) A proposed model showing how loss of BAF155 and BAF170 in dcKO mutants controls epigenetic and neural gene expression programs in proliferation and neuronal differentiation of the pallium in late developmental stages.

Values are presented as means ± SEMs (∗p < 0.05; ∗∗p < 0.01, ∗∗∗p < 0.005; ∗∗∗∗p < 0.0001). Experimental replicates (n) = 4 (E, F, and G). Scale bars represent 100 μm (C, D, and F) and 50 μm (C).