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. Author manuscript; available in PMC: 2019 Apr 14.
Published in final edited form as: Bioorg Med Chem Lett. 2017 Dec 8;28(3):470–475. doi: 10.1016/j.bmcl.2017.12.016

Table 1.

[3]CFT binding to WT, R445E, E428R, and R445E-E428R DAT and its inhibition by DA

[3H]CFT binding WT R445E E428R R445E-E428R
Kd (nM) 13.1 ± 2.62 67.4 ± 7.26* 35.2 ± 2.14* 4.83 ± 0.83
Bmax (pmol/mg) 1.04 ± 0.14 3.27 ± 0.63 2.50 ± 0.30* 0.56 ± 0.11
DA Ki (μM) 3.83 ± 0.579 22.4 ± 4.7** 21.8 ± 10.2** 3.66 ± 0.53

Values are mean ± SE; n = 4 for [3H]CFT Kd and Bmax values and n = 5–6 for DA Ki values.

*

P<0.05,

**

P<0.01 compared with wild-type (WT) (one-way ANOVA followed by Dunnett multiple comparisons with WT).