Figure 7. miR-21−/− macrophages exerted stronger anti-tumor effects than their WT counterparts after co-culture with B16 cells.
(a) Schematic representation of in vitro macrophage cultivation and implantation with B16 tumor cells. (b) Representative images of B16 tumors 16 days post-inoculation; n = 5 for each group. (c) Tumor weights. (d) Tumor volumes; data were analyzed with one-way ANOVA. (e) Representative graphs of flow cytometry analysis of infiltrating M1 and M2 TAMs within tumors. (f, g, h, i) Infiltrating TAMs, CD8+ T cells, M1 and M2 TAM fractions and the M1: M2 ratio; n = 4 for each group. *P ≤ 0.05, **P ≤ 0.01, n.s., not significant.