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. Author manuscript; available in PMC: 2019 Jun 8.
Published in final edited form as: ACS Infect Dis. 2018 Mar 23;4(6):1007–1018. doi: 10.1021/acsinfecdis.8b00017

Figure 1. Recombineering strategy for allelic exchange using positive selection.

Figure 1

After transformation of a vector containing flanking homology arms to direct recombination to a particular chromosomal locus, a first crossover event leads to integration of the vector into the chromosome. The vector recombines out through a second crossover event, producing a mutant allele or regenerating the wildtype allele depending on whether the upstream or downstream homology region is used for recombination. pKFC-mediated deletion of chromosomal genes utilizes this recombineering strategy.

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