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. Author manuscript; available in PMC: 2019 Jun 8.
Published in final edited form as: Circ Res. 2018 Apr 27;122(12):1703–1715. doi: 10.1161/CIRCRESAHA.118.312978

Table 6.

Biorepository

Biologic Sample # Sample details Potential ancillary analyses on stored (frozen) samples
Bone marrow n=144
(all patients)
Fresh BM, flash frozen buffy coat, cryopreserved buffy coat, cryopreserved BM supernatant In vivo and in vitro studies, RNA, DNA, protein
BM-MNCs n=72,
(placebo & CPC patients)
Cryopreserved MNCs In vivo and in vitro studies, RNA, DNA, protein
Excess cell product n=144
(3 active groups)*
10-30 million MSCs, 0.5-1 million CPCs In vivo and in vitro studies, RNA, DNA, protein
Cell culture medium n=144,
(3 active groups)*
up to 10 12-ml aliquots Secretome (microparticles/ exosomes, miRNAs, cyto/chemokines, GFs)
Peripheral Blood n=144
(all patients)
Flash frozen buffy coat and plasma, cryopreserved buffy coat and plasma In vivo and in vitro studies, RNA, DNA, protein, secretome (microparticles/ exosomes, miRNAs, cyto/chemokines, GFs)
Explanted Heart
(as available)
Unknown 10 cardiac regions, formalin fixed or flash frozen RNA, DNA, protein, histology,
*

One product/MSC patient, 1 product/CPC patient; 2 products/MSC + CPC patient

Analyses require ancillary study application and funding source outside scope; samples are stored in such a way as to realize these potential future analyses