Table 6.
Biorepository
| Biologic | Sample # | Sample details | Potential ancillary analyses on stored (frozen) samples† |
|---|---|---|---|
| Bone marrow | n=144 (all patients) |
Fresh BM, flash frozen buffy coat, cryopreserved buffy coat, cryopreserved BM supernatant | In vivo and in vitro studies, RNA, DNA, protein |
| BM-MNCs | n=72, (placebo & CPC patients) |
Cryopreserved MNCs | In vivo and in vitro studies, RNA, DNA, protein |
| Excess cell product | n=144 (3 active groups)* |
10-30 million MSCs, 0.5-1 million CPCs | In vivo and in vitro studies, RNA, DNA, protein |
| Cell culture medium | n=144, (3 active groups)* |
up to 10 12-ml aliquots | Secretome (microparticles/ exosomes, miRNAs, cyto/chemokines, GFs) |
| Peripheral Blood | n=144 (all patients) |
Flash frozen buffy coat and plasma, cryopreserved buffy coat and plasma | In vivo and in vitro studies, RNA, DNA, protein, secretome (microparticles/ exosomes, miRNAs, cyto/chemokines, GFs) |
|
Explanted Heart (as available) |
Unknown | 10 cardiac regions, formalin fixed or flash frozen | RNA, DNA, protein, histology, |
One product/MSC patient, 1 product/CPC patient; 2 products/MSC + CPC patient
Analyses require ancillary study application and funding source outside scope; samples are stored in such a way as to realize these potential future analyses