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. 2018 May 17;10(6):1991–2004. doi: 10.1016/j.stemcr.2018.04.020

Figure 1.

Figure 1

Optopatch Reports Firing Patterns of iPSC-Derived Motor Neurons in a Model of ALS

(A) Pipeline for disease modeling with optical electrophysiology.

(B) Timeline of motor neuron differentiation, gene transduction, maturation, and measurement.

(C) Top: domain structure of Optopatch constructs. Bottom: images of an iPSC-derived motor neuron expressing both CheRiff-EGFP and QuasAr2-mOrange2.

(D) Simultaneous fluorescence and patch-clamp recordings of spiking in iPSC-derived motor neurons under optical stimulation. Left: images from mutant and genome-corrected controls. Right: fluorescence (red, black) and voltage (blue). Illumination protocols are shown above.

All scale bars, 10 μm. See also Figure S1.