DC Activation Induced by CD40Lhigh CD4+ iPS-T Cells
(A) Flow cytometry profiles of the surface molecules on DCs. Vehicle- or b3a2-peptide-pulsed DCs were cultured for 24 hr with the CD40Lhigh CD4+ and CD40Llow CD4+ population at a DC/CD4+ iPS-T cell ratio of 5:1. Relative fluorescence intensity (RFI) (%) of indicated molecule is calculated as follows: RFI (%) = 100 × (RFI of b3a2 peptide-treated cells)/(RFI of vehicle-treated cells).
(B) Cytokine production by DCs co-cultured with the indicated population. Cytokines in the culture supernatant were measured by a bead-based multiplex immunoassay. The indicated population (1 × 104) was co-cultured for 24 hr with autologous DCs (2.5 × 104) that had been prepulsed with b3a2 peptide (10 μM). The original CD4+ Th1 clone (SK) served as a control.
(A and B) Data shown are the means ± SD of triplicate cultures and are representative of two independent experiments.