Figure 3.

DC Activation Induced by CD40L^high CD4⁺ iPS-T Cells

(A) Flow cytometry profiles of the surface molecules on DCs. Vehicle- or b3a2-peptide-pulsed DCs were cultured for 24 hr with the CD40L^high CD4⁺ and CD40L^low CD4⁺ population at a DC/CD4⁺ iPS-T cell ratio of 5:1. Relative fluorescence intensity (RFI) (%) of indicated molecule is calculated as follows: RFI (%) = 100 × (RFI of b3a2 peptide-treated cells)/(RFI of vehicle-treated cells).

(B) Cytokine production by DCs co-cultured with the indicated population. Cytokines in the culture supernatant were measured by a bead-based multiplex immunoassay. The indicated population (1 × 10⁴) was co-cultured for 24 hr with autologous DCs (2.5 × 10⁴) that had been prepulsed with b3a2 peptide (10 μM). The original CD4⁺ Th1 clone (SK) served as a control.

(A and B) Data shown are the means ± SD of triplicate cultures and are representative of two independent experiments.