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. 2018 May 24;10(6):1935–1946. doi: 10.1016/j.stemcr.2018.04.025

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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CD40L^high CD4⁺ iPS-T Cells Reduced NK Cell-like Cytotoxicity

(A) Left panel: cytotoxic activity of iPS-T cells to THP-1 cells. Center panel: Mock iPS-T cells were treated with 10 nM concanamycin A (CMA) to block perforin. Right panel: Indicated Abs blocking receptor/ligand interactions were added. Cytotoxicity of iPS-T cells to THP-1 cells at an effector/target (E:T) ratio of 2.5:1.

(B) Left panel: representative flow cytometry profiles of TCR-Vβ22, CD4, CD40L, DNAM-1, and NKG2D on the indicated population. Right panel: cytotoxic activities of the indicated population to HLA-DR9-expressing THP-1 cells loaded with vehicle or b3a2 peptide (5 μM).

(A and B) Cytotoxicity was measured by ⁵¹Cr-release assay for 4 hr at the indicated E:T ratios. Data are representative of two independent triplicate experiments.