Figure 2.

RA Is Sufficient to Stimulate Adult Hippocampal NSPC Proliferation and HIF1α Mediates This Effect In Vitro

(A–G) Quantification (A) of labeling index (LI) in cultures of NSPCs. RT-PCR for (B) Hif1a and (D) Vegfa. ELISA quantification of (C) HIF1α and (E) VEGFA protein levels. Transcriptional inhibitor actinomycin D ablates RA-induced increase in (F) Hif1a gene expression and (G) HIF1α protein level.

(H) Quantification of LI for NSPC cultures treated with vehicle, RA, echinomycin, SU 5,408, RA + echinomycin or RA + SU 5,408.

(I) Quantification of LI for NSPC cultures treated with vehicle, DMOG, SU 5408, or DMOG + SU 5408.

Data represented as means ± SEM, ^∗,#p ≤ 0.05, ^{∗∗∗,###}p ≤ 0.001, n = 3.