Figure 4.

RA-HIF1A Regulates Adult Hippocampal NSPC Proliferation In Vivo

(A) Schematic of experimental paradigm.

(B) Quantification of dentate gyrus (DG) volume in vehicle-, DS-, DS + DMOG-, or DMOG-treated mice.

(C and D) ELISA for HIF1α and VEGFA protein in hippocampus from vehicle-, DS-, DS + DMOG-, or DMOG-treated mice.

(E–H) Quantification of total number of NSPC subtypes type 1 (E), type 2a (F), type 2b (G) and type 3 (H) across all treatments.

(I) Confocal images showing EdU (white) labeling in the SGZ across all treatments.

(J–M) Quantification of percentage proliferating NSPC subtypes type 1 (J), type 2a (K), type 2b (L) and type 3 (M) in vehicle-, DS-, DS + DMOG-, or DMOG-treated mice.

Scale bar, 100 μm. Data represented as mean ± SEM, ^∗p ≤ 0.05, ^∗∗,##p ≤ 0.01, ^{∗∗∗,###}p ≤ 0.001, n = 3.