Table 2.

Carbohydrate active enzymes (CAZy) encoded in the P. saccharofermentans str. M3/6^T genome.

Substrate target	Enzyme name	ECa number	Familyb
Pectin	Pectinesterase	3.1.1.11	CE8
	Exo-poly-α-d-galacturonosidase	3.2.1.82	GH28
	α-l-rhamnosidase	3.2.1.40	GH33
	α-l-rhamnosidase	3.2.1.40	GH78
	Rhamnogalacturonyl hydrolase	3.2.1.172	GH105
	Pectate lyase superfamily protein		GH28
Arabinogalactan	Arabinogalactan endo-β-1,4-galactanase	3.2.1.89	GH53
	Putative Galactan endo-1,6-β-galactosidase	3.2.1.164	GH30
Arabinan (Hemicellulose)	Putative α-l-arabinofuranosidase	3.2.1.55	GH43
	α-l-arabinofuranosidase	3.2.1.55	GH51
	β-l-arabinofuranosidase	3.2.1.185	GH127
	Arabinan endo-1,5-α-l-arabinosidase	3.2.1.99	GH43
Xylan (Hemicellulose)	Xylan 1,4-β-xylosidase	3.2.1.37	GH43
	Xylan 1,3-β-xylosidase	3.2.1.72
	Acetyl xylan esterase	3.1.1.72
Mannan (Hemicellulose)	β-mannanase	3.2.1.78	GH26
	Putative mannan endo-1,6-α-mannosidase.	3.2.1.101	GH76
Lichenan	Licheninase	3.2.1.73	GH16
Cellulose	Cellulase	3.2.1.4	GH5
	Cellulose 1,4-β-cellobiosidase	3.2.1.91	GH9
	β-glucosidase	3.2.1.21	GH3
Chitin	Chitinase	3.2.1.14	GH18
	β-N-acetylhexosaminidase	3.2.1.52	GH20
	N-acetylglucosamine kinase	2.7.1.59
	Putative β-hexosaminidase	3.2.1.52	GH3
Starch	α-amylase	3.2.1.1	GH13
	Putative glucan 1,4-α-glucosidase	3.2.1.3	GH15
	Putative α-amylase	3.2.1.1	GH57
	4-α-glucanotransferase	2.4.1.25
	Glycogen Phosphorylase	2.4.1.1	GT35
Pullulan	Neopullulanase	3.2.1.135	GH13
Fructan	Fructan β-fructosidase	3.2.1.80	GH32
	β-fructofuranosidase	3.2.1.26	GH32

Enzymes encoded in the P. saccharofermentans str. M3/6^T genome were compared against the HMM profile-based database dbCAN using hmmsearch in the HMMER software package. Results were manually curated considering best BLAST hits, alignments, and e-values. In addition, PRIAM profiles were used for classifications.

^aEnzyme Commission number.

^bCAZy families.