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. 2018 Jun 8;9:2253. doi: 10.1038/s41467-018-04787-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Strains of cluster 13 grow less well than other strains of C. albicans in different media at different temperatures. Violin plots of growth size scores performed using Iris by grouping isolates by clusters (only the most represented clusters are shown, i.e., those with >10 individuals: clusters 1, 2, 3, 4, 11 and 13) on vaginal-simulative medium (VSM) and YPD; For saliva simulative medium (SSM), we calculated colony size as mean of two perpendicular measures of diameters using ImageJ. Horizontal lines in the boxplots represent the median, vertical ones the length between upper and lower quartiles. Dots represent the outlier values. Some strains showed very poor growth on VSM, preventing measurement of colony size. These strains were not included in the figure. All experiments were performed in duplicate. In all conditions (different media and different temperatures), cluster 13 isolates are significantly less fit than other strains of C. albicans (ANOVA analyses followed by post-hoc comparisons using TukeyHSD tests (see Supplementary Data 5)). VSM: vaginal-simulative medium, SSM: saliva-simulative medium, YPD: yeast peptone dextrose medium