Fig. 5.

Morphogenesis of 189-positive larval LNs during pupal development. a–d The same labeling strategy as in Fig. 4c was used to examine the morphologies of 189Y-positive larval LNs in 24 h APF (n = 50 SCs/154 ALs) a, 36 h APF (n = 51 SCs/211 ALs) b, 48 h APF (n = 51 SCs/138 ALs) c and 72 h APF (n = 32 SCs/121 ALs) d pupal brains. Brains were stained with HA and DNcad (not shown) to visualize neurons and neuropil, respectively. The larval LNs were observed to have processes that densely covered the medioventral region and the dorsolateral region of the AL (a₁–d₁), that are mostly concentrated in the medio-central portion of the AL (a₂–d₂), that innervate the centrolateral portion of the AL (a₃–d₃), or that are sparse and irregular in the AL (a₄–d₄). LNs from each developmental stage were categorized by the similarity of their neurite density, coverage, and occupied regions. LNs shown in a₁–d₁, a₂–d₂, a₃–d₃, and a₄–d₄ are likely, but not necessarily, of the same type. ALs are contoured by yellow dashed lines. Yellow arrowheads indicate LN soma. Asterisk denotes processes from non-LN cells that are not in the AL. Scale bars, 20 μm. e Hierarchical clustering of the innervation profiles of individual LNs in 72 h APF pupal brains (n = 29) (top) or adult brains (n = 12) (bottom). These LNs showed heterogeneous innervation patterns, but fall into four broad types