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The amount and characteristics of mPCR components.
| The utilized components | DNA Template | Taq DNA polymerase | Reverse primer (Each) | Forward primer (Each) | dNTPs | MgCl2 | PCR Buffer | H2O |
|---|---|---|---|---|---|---|---|---|
| Density | - | 5 U/µl | 20 µM | 20 µM | 10 mM | 50 mM | 10× | - |
| Amount (ml) | 2 | 0.3 | 1.5 | 1.5 | 2 | 1.8 | 2.5 | 7.4 |
