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. 2018 Jun 8;8:8763. doi: 10.1038/s41598-018-27037-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characteristics of droplets and particles. (A) Light microscope image shows consecutive droplets within the particle generator chip connected to syringe pumps. Acrylamide droplets were then polymerized into polyacrylamide particles. (B–E) Fluorescence micrographs show polyacrylamide particles containing fluorescein-modified DNA. (B) Microparticles were generated by a microfluidic chip with cholesterol modified DNA and (C) without cholesterol-modified DNA. Polyacrylamide microparticles were also generated using mechanical homogenization (D) containing DNA with cholesterol and (E) without cholesterol. (F) Histograms show the distribution of cholesterol-modified DNA at the surface of particles manufactured using the microfluidic generator and (G) those manufactured using mechanical homogenization.