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. 2018 Jun 8;8:8759. doi: 10.1038/s41598-018-27053-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Intrahippocampal infusion of rapamycin 15 minutes before test does not alter neuronal internal state of the animals. Rats were bilaterally infused with rapamycin (Rapa) or saline (Veh) into hippocampus CA1 at two different time points: immediately after training (TR) and 15 min before a test (TS) session carried out 24 h after the TR. **p < 0.01, for Veh-Veh vs. Veh-Rapa, Veh-Veh vs. Rapa-Veh and Veh-Veh vs. Rapa-Rapa groups, one-way ANOVA followed by Newman-Keuls Comparison Test; n = 7-8. Data are expressed as mean ± SEM of TR or TS session step-down latency at 24 h after TR.