Fig. 1.

MIF is required for the release of IL-1 family cytokines. Primary murine WT and Mif^−/− BMDMs were left untreated (control), primed with LPS (10 ng ml⁻¹), or primed with LPS followed by nigericin (5 µM) treatment for 1 h. Levels of a IL-1α, b IL-1β, and c IL-18 in cell culture supernatants were assessed by ELISA. Primary WT BMDMs were left untreated, primed with LPS alone (10 ng ml⁻¹), primed with LPS, and then treated with or without COR123625 (50 µM) for 2 h before the addition of nigericin (5 µM) for 1 h. Levels of d IL-1α, e IL-1β, and f IL-18 in cell culture supernatants were assessed by ELISA. C57BL6/J mice were injected intraperitoneally with vehicle control (saline), LPS alone (2 mg kg⁻¹) or COR123625 (20 mg kg⁻¹) in combination with LPS for 2 h. Serum levels of g IL-1β, h IL-18, and i IL-6 were measured by ELISA. Data are expressed as means ± SEM, n = 3–4 mice per group. *P < 0.05, ***P < 0.005, ****P < 0.001, one-way ANOVA with a correction provided by the Tukey's multiple comparisons test