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. 2018 Mar 27;28(6):670–685. doi: 10.1038/s41422-018-0023-9

Fig. 1.

Fig. 1

SUMO inhibits phosphorylation of Pol II Ser2 and selectively sumoylates CDK9. a The levels of Ser2P, Ser5P, Pol II, acH3 and acH4 were determined by WB analysis upon ectopic expression of PIAS1 and PIAS1m with SUMO-1 in HeLa cells. b IF analysis showing the effect of ectopically expressed PIAS1 and PIAS1m with or without SUMO-1 on Ser2P of Pol II CTD. c WB analysis for known proteins involved in the phosphorylation of Ser2 of the Pol II CTD. Sumoylation was detected only in CDK9 upon ectopic expression of PIAS1/SUMO-1